ABSTRACT
Ovarian and follicle transplantation may preserve fertility in young cancer survivors. In this study, we have transplanted preantral follicles using fibrin gel as a carrier and fibrin gel supplemented with platelet lysate [PL] as a rich source of angiogenic and growth factors. The purpose of this study was to evaluate the role of fibrin gel and PL in follicle transplantation. In this experimental study, ovaries were taken from 14-day-old Naval Medical Research Institute [NMRI] mice. Preantral follicles were dissected from the ovaries and encapsulated into fibrin gel supplemented with 5, 10, 15 or 20% PL, then transplanted back into the same donor mice. Fibrin gels supplemented with PL that contained preantral follicles were placed in a subcutaneous pocket in the back of the neck of the recipient, donor mouse [the same mouse that follicles were collected]. After 14 days the grafts were processed and embedded in paraffin blocks, then serially sectioned for histological evaluation. We counted the follicles and classified them according to stage [preantral or antral]. Data were presented as mean +/- standard error of mean [SEM] and analysed by analysis of variance [ANOVA] and the Kruskal-Wallistest. The mean percentage of recovered follicles encapsulated and transplanted in each group were 33.30 +/- 2.47 [fibrin gel], 31.96 +/- 1.90 [fibrin gel+5% PL], 34.02 +/- 2.44 [fibrin gel+10% PL], 48.31 +/- 2.06 [fibrin gel+15% PL] and 17.60 +/- 2.79 [fibrin gel+20% PL]. There was a significant increase in the recovery rate of grafted follicles with fibrin gel+15% PL [48.31%; p<0.001]. The percentage of preantral follicles showed no significant difference in all groups [p<0.05]. The percentage of antral follicles showed a significant decrease in follicles grafted with fibrin gel+20% PL when compared to the other groups [11.77%; p<0.005] but no significant difference was observed in the other groups. The use of PL in follicle transplantation can improve ovarian follicular survival rate
ABSTRACT
Recent reports have suggested that cytomegalovirus [CMV] infection may contribute to risk of cardiovascular disease. However, relationship between CMV infection and unstable angina [UA] is controversial and studies about this subject in Iran and even region are lacking. The aim of this study was to determine whether unstable angina is related to seropositivity to chronic cytomegalovirus infection. We measured serum CMV IgG levels in a case control study participants in CCU in Razi Hospital, Ahvaz, Iran, from 2004 to 2005. Blood samples were drawn during study period from 96 patients [mean age 56 years] with UA according to American Heart Association Criteria and from 96 participants free of cardiovascular disease [mean age 58 years] and stored at -20 Degree C. Blood samples of patients were undertaken for investigating the specific anti CMV-IgG by ELISA method. Data were analyzed in SPSS 11.5 by using chi square test, odds ratios [OR] with 95% confidence intervals [CI]. Ninety three percent of patients with unstable angina and 96.7% in the control group presented a positive anti CMV-IgG. Odds ratio was 0.52 with 95% CI: 0.10 to 2.42. There was no significant correlation between CMV-IgG positivity and unstable angina [P>0.05]. There was also no differences in CMV-IgG positivity in clinical groups of UA [P>0.05]. The relationship between seropositivity of CMV-IgG and unstable angina has been restituted by the results of this study. However, further population based cohort studies for relationship between CMV infection and coronary artery disease must be conducted
Subject(s)
Humans , Male , Female , Angina, Unstable/virology , Chronic Disease , Case-Control Studies , Immunoglobulin G , Enzyme-Linked Immunosorbent AssayABSTRACT
To compare two drugs regimen, doxycycline-rifampicin and doxycycline-cotrimoxazol in the treatment of brucellosis patients. It is a comparative clinical study conducted in Ahwaz a city southwest Iran, from April 2004 to January 2006. This study was conducted on 102 nomads' patients with brucellosis. The diagnostic criteria were the finding of >/= 1/80 [Wright] with a 2 mercaptoethanol [2 ME] >/= 1/40, in association with compatible clinical findings [back pain, sweating and fever]. Patients were enrolled into the two antimicrobial therapy groups, doxycycline plus rifampicin [DR group] and doxycycline plus co-trimoxazole [DC group]. The data in the two groups were statistically compared with SPSS by chi square test. Failure of treatment was seen in one [1.94%] and 5 [9.81%] cases treated in the DC group and DR group, respectively. Relapse was seen in three cases [5.88%] treated in the DC group and in six cases [11.76%] treated in the DR group. Failure of treatment plus relapse was seen in four [7.84%] and 11 [21.56%] cases treated in the DC group and DR group, respectively [p <0.05]. Risk for relapse and failure of treatment in DR group was 2.75 times higher than DC group. This study showed that co-trimoxazole plus doxycycline regimen has a better therapeutic effect than doxycycline plus rifampicin regimen